CURRICULUM VITAE

JOANNA KATHERINE KRUEGER

Associate Professor, Department of Chemistry

University of North Carolina at Charlotte

9201 University City Blvd. Charlotte, NC 28223-0001

phone: (704) 687-4913 fax: (704) 687-3151 JKKruege@uncc.edu

Education

October 1991 Ph. D. Chemistry. Princeton University

Thesis advisor: Dr. C. E. Schutt, Chemistry Department

Thesis title: “Structure Function Studies of the Methylesterase Involved in Bacterial Chemotaxis”.

Research: The active site and enzymatic mechanism of CheB, an important methylesterase in bacterial chemotaxis, was studied utilizing selected-site mutagenesis, protein purification, crystallization and kinetic assays.

June 1988 M. A. Chemistry. Princeton University

June 1985 B. A. Chemistry (A.C.S. certified). Kalamazoo College

Honors and Awards

2003-08 National Science Foundation CAREER Award

2003-05 Research Corporation Cottrell College Science Award

2001-02 Oak Ridge Associated Universities Ralph E. Powe Junior Faculty Enhancement Award

1994-96 National Research Service Award- A two-year post-doctoral fellowship awarded by the National Heart, Lung and Blood Institute.

1993-94 Cardiology Training Fellowship- National Institutes of Health

1982-85 F. W. and Elsie L. Heyl Scholarship- Full tuition paid undergraduate scholarship

Research Experience

8/05- present Associate Professor. University of North Carolina at Charlotte. Chemistry Department

8/99-8/05: Assistant Professor. University of North Carolina at Charlotte. Chemistry Department

Research Focus: Structural information on biomolecular associations using the techniques of small-angle X-ray and neutron scattering, selected-site mutagenesis and spectroscopy (FTIR, CD, UV-VIS); and visualized through the use of Monte Carlo molecular modeling.

8/95-8/99 Post-doctoral Associate (NIH fellow, 1995-96). Los Alamos National Laboratory

Research Advisor: Dr. J. Trewhella, Chemical Science and Technology Division

Research: Utilized small-angle X-ray and neutron scattering techniques to study the structures of calcium calmodulin complexed with an enzymatically active myosin light chain kinase as well as other proteins and protein complexes in solution.

7/92-8/95 Post-doctoral Associate (NIH fellow, 1993-95). University of Texas Southwestern Medical Center at Dallas

Research Advisor: Dr. J. T. Stull, Physiology Department

Research: Used selected-site mutagenesis and kinetic analyses to study the regulatory mechanism for smooth muscle myosin light chain kinase cloned and purified from baculovirus.

Research Experience (cont’d)

7/91-7/92 Research Assistant at U.T. Southwestern Medical Center

Supervisor: Dr. R. S. Williams, Departments of Cardiology and Internal Medicine

Research: Functional studies involving the use of tissue culture, southern blotting, selected-site mutagenesis and molecular biology to study human myoglobin transcription.

1/85-4/85 Senior Research Student at Texas A & M University

Advisor: Dr. T. O. Baldwin, Department of Biochemistry and Biophysics

Research: Cloning, Maxim-Gilbert sequencing of the bacterial luciferase gene from V. fisheri.

3/83-9/85 Research Assistant at KALSEC (KALamazoo Spice Extraction Co.)

Advisor: Dr. R. J. Cook, Employer: Mr. Paul Todd; owner

Research: Developed and tested several herb and spice extracts for their potential as a natural food preservative. A marketable product was obtained.

Research Support

1999- present

Internal

2000 UNC Charlotte Junior Faculty Summer Fellowship Program award ($3500) [J. Krueger, PI]

2000-2001 UNC Charlotte Faculty Research Support grant ($5000) [J. Krueger, PI]

2001 UNC Charlotte Junior Faculty Summer Fellowship Program award ($3500)

[J. Krueger, PI]

2002 Betty J. and J. Stanley Livingstone Faculty Enhancement Award ($7200)

2003-2004 UNC Charlotte Faculty Research Support grant ($6000) [J. Krueger, PI]

2006-2007 UNC Charlotte Senior Faculty Research Grant ($6,000) “Inhibition and Regulation of Phosphodiesterase-5” [J. Krueger, P. I]

External

2000-2002 North Carolina Biotechnology Center Academic Research Initiation Grant ($55,000) “Protein conformational changes associated with regulation of the actin cytoskeleton as studied by small-angle solution scattering”. [J. Krueger, PI]

2000-2001 North Carolina Super Computing Center Computational Science Faculty Account; 200 CPU Hours on SGI origin 2000. [J. Krueger, PI]

2001-2002 Oak Ridge Associated Universities Ralph E. Powe Junior Faculty Enhancement Award ($5,000 +$5,000 matching from UNC Charlotte) “The structure of gelsolin in complex with an actin dimer as studied by neutron scattering”.

[J. Krueger, PI]

2003 NIST Center for Neutron Research (Dept. of Commerce) Competitive proposal accepted for 3 days of neutron beam time on beamline NG3.

2003-2005 Research Corporation Cottrell College Science Award ($39,718 +$26,150 matching from UNC Charlotte) “Distance Constraints For Building An Atomic Model of Gelsolin:2Actin Complex Determined By Chemical Cross-Linking and Peptide Mapping”. [J. Krueger, PI]

2003- 2008 National Science Foundation CAREER Award ($571,677) “Integrated Structural Biology Approach to Building Atomic Models Of Actin Complexes”. [J. Krueger, PI]

2005- 2007 Brookhaven National Lab National Synchrotron Light Source (NSLS, Department of Energy) competitive proposal "SAXS on gelsolin:actin complexes." accepted for access to beamline X21. Submitted in September 2004 [J. Krueger, PI].

2006-2008 Brookhaven National Lab National Synchrotron Light Source (NSLS, Department of Energy) competitive proposal " SAXS of complex between HIV-1 GP120 and sCD4." accepted for access to beamline X21. Submitted in September 2005; Active for January 2006 – January 2008. [J. Krueger, PI].

2006 NIH AIDS Reagent Program. Non-competitive proposal for free access to AIDS-related proteins for approved research purposes. [J. Krueger, P.I]

2006 Argonne National Laboratory Advanced Photon Source (APS, Department of Energy) competitive proposal “SAXS on F-actin:gelsolin interactions”. Accepted for access to BioCAT beamline. Submitted Spring 2006 for Fall 2006 access. [J. Krueger, PI].

Invited Talks and Contributed Talks at National Meetings

“Small-angle Scattering: Solutions in Protein Structural Analysis”.

March 1, 2002: Oak Ridge Associated Universities’ 57^th annual Meeting of the Council of Sponsoring Institutions and Mini-Symposium on Neutron Scattering. February 28 – March 1, 2002. Oak Ridge, TN.

March 21, 2002: University of North Carolina Pembroke. Chemistry Department.

March 26, 2002: Syracuse University. Chemistry Department.

April 8, 2002: Workshop on Using Neutrons to Probe Structure and Dynamics in Biological Systems, Joint Institute for Neutron Science. April 8 – 10, 2002. Oak Ridge, TN.

September 13, 2002: Appalachian State University. Chemistry Department.

March 27, 2003: Eastern Carolina University. Chemistry Department.

April 26, 2003: 2^nd Annual Low Energy Neutron Source Users Meeting. April 25 – 26, 2003. Indiana University.

May 21, 2003: National Syncrotron Light Source Annual Users Meeting. Advanced Photon Source Workshop. Brookhaven National Laboratory.

September 24, 2003: Neutron Scattering For Chemistry and the Chemistry/Biology Interface (NSF CHEMBIO) Workshop. Sept. 23- 26, 2003. Florida State University.

November 11, 2003: University of South Carolina. Chemistry Department’s Nanocenter Series Talk http://nanonet.research.sc.edu/videos/kreuger_files/default.htm

March 8, 2005: University of Maryland Baltimore County. Chemistry Department

March 18, 2005: Davidson College. Chemistry Department

“A SANS Contrast Variation Study to Elucidate Structural Requirements for the Initial Recognition Step between Myosin Light Chain Kinase and Its Activator Protein, Calmodulin”.

June 8, 2004: 2^nd American Conference of Neutron Scattering. June 6–9, 2004 Baltimore, MD.

“SAXS/SANS Studies Of Calmodulin Interaction With Skeletal Myosin Light Chain Kinase Catalytic Domain”.

Oct. 22, 1999: The Eleventh International Symposium on Calcium-Binding Proteins and Calcium Function in Health and Disease October 19 -23, 1999. Kisarazu, Japan.

“The Solution Structure of Calmodulin and a Functional Myosin Light Chain Kinase in the Activated Complex: A Study Using Small-angle X-ray and Neutron Scattering with Contrast Variation”.

Oct. 30, 1996: Interdisciplinary Structural Biology Group at Los Alamos National Laboratory.

Mar. 4, 1997: Contributed talk at the Biophysical Society Meeting. New Orleans, LA.

Apr. 11, 1997: University of Texas Southwestern Medical Center@Dallas, Physiology Department, April 11, 1997.

Aug. 21, 1997: Contributed talk at the ASBMB satellite meeting on Cellular Regulation by Protein Phosphorylation in Seattle, WA.

"Small-angle Solution Scattering Reveals Structural Details on an Activation Mechanism that is Key to Calcium's Control of Muscle Contraction"

Nov. 16, 1998: Department of Biochemistry and Molecular Biology, Milton S. Hershey Medical Center, Pennsylvania State University.

Nov. 20, 1998: Department of Chemistry and Biochemistry, San Francisco State University.

Dec. 11, 1998: Department of Biochemistry, University of Kentucky College of Medicine.

Dec. 16, 1998: Department of Biochemistry, Virginia Tech.

Jan. 14, 1999: Department of Biochemistry and Molecular Biology, University of Tennessee.

Mar. 3, 1999: Chemistry Department, Rensselaer Polytechnic Institute.

Mar. 15, 1999: Joan and Sanford I. Weill Medical College of Cornell University, New York.

Mar. 29, 1999: Chemistry Department, University of North Carolina at Charlotte.

Teaching Experience

UNC Charlotte CHEM 4165/5165- Principles of Biochemistry I [F ’99; F ’00; F’04; F’06]

CHEM 4171/5171- Biochemical Instrumentation [S ’00; S’06]

CHEM 6101/8101- Biochemical Principles [F ’99; F ’00; F ’01; F ’03; F’05]

CHEM 6165/8165- Advanced Biochemistry [S ’03; S ‘05]

CHEM 4166/5166- Principles of Biochemistry II [S ’01; S ’02; S ’04]

CHEM 4165L- Principles of Biochemistry I Laboratory [F ‘01]

CHEM 4695 and CHEM4696- Mentor for students in this Chemistry Seminar Course [a total of 24 students: (3) F ’00; (3) S ’01; (1) S ’02; (1) S ’03; (2 +1H) F’03; (4 +1H) S’ 04; (3) F ’04; (1) S’05; (2) F’05; (2) S ‘05]

CHEM 6090/ 8090- Nanoscale Science&Technology (Guest lecture S ’04)

CHEM 4900- Directed Undergraduate Research (> 25 students)

CHEM 6900- Directed Graduate Research

· Paul Perryman “Small-angle x-ray scattering reveals Ca2+-induced activation of the actin-binding protein gelsolin” (MS ’01)

· Kim Chichester “Preparation of actin from Dictyostellium discoideum for small-angle x-ray scattering experiments”. (Incomplete; left UNCC ‘03)

· Shani Smith “Small-angle x-ray scattering on gelsolin:actin:actin complex”. (Incomplete; left UNCC ’02)

· Nidhi Modi “Investigation into the deuteration level of proteins using electrospray ionization mass spectroscopy and NMR”. (MS ‘05)

· Matthew Paine “Shape restoration modeling of SAXS data on Ca²⁺ titration of gelsolin” (MS ‘06)

· Malgorzata Tracka “Following the secondary structure rearrangements with gelsolin and actin upon complex formation using Circular Dichroism and Fourier Transform Infrared Spectroscopy”. (MS ‘06)

· Jonathan Osborne “Allosteric regulation of phosphodiesterase-5 by divalent cations, cGMP and Viagra; domain structure rearrangements as observed by small-angle X-ray scattering”. (MS ‘06)

· Suzanne Wolfe “Chemical cross-linking and peptide mapping on f-actin dimers” (Incomplete; left UNCC ‘06)

· Chris Boone “Toward a Structural Understanding of the Viral Entry Mechanism of HIV-1”.

BIOTechnology Ph. D.

· (Laboratory Rotation) Mike York “Computational Calculations for SAXS on Gelsolin/Actin” F ‘99

· Monalisa Sunkara “Small-angle neutron scattering investigation on specifically deuterated actin bound to gelsolin” (Expected completion ’08-’09)

Technical Expertise

Molecular Biology: Gene subcloning, plasmid purification, nucleic acid sequencing, selected-site mutagenesis, polymerase chain reaction (PCR), tissue culture, bacterial and baculovirus expression systems

Biochemical: Protein purification and crystallization, enzymology and kinetic analyses, western and southern blots, molecular modeling, capillary electrophoresis

Spectroscopic: Circular dichroism, Fluorescence and UV-Vis spectroscopy, small-angle X-ray and neutron scattering

Computational: Familiarity with Unix, Mac OS and DOS operating systems; Pascal, Fortran, C and C++ programming languages; Iris Workstations and molecular viewing and building with INSIGHTII program package

Professional Memberships

Biophysical Society (BPS); The Protein Society (FASEB); American Association for Advancement of Science (AAAS); American Crystallography Association (AC A); American Chemical Society (ACS)

American Society for Biochemistry and Molecular Biology (ASBMB)

Service Activities

National Committees

Member (elected) of the Editorial Board for ASBMB’s Journal of Biological Chemistry

Member (invited) of the Advanced Photon Source (APS) General User Small Angle X-ray Scattering (SAXS) Proposal Review Panel (PRP). Term of service is from Oct. 2005 – Oct. 2007.

Invited by LANSCE (Los Alamos Neutron Science Center) and DOE managers to attend, and to provide comments and advice at, a LANSCE Workshop in Washington, DC, June 8-9, 2005.

Member (appointed), National Institute of Standards and Technology Center for Neutron Research (NCNR) program advisory committee (beginning Sept. 2004)

Observer (invited), Experimental Facilities Advisory Committee Meeting, Spallation Neutron Source (SNS) April 14- 16, 2004

Member (elected for 2003-2005 term), SHUG (Spallation Neutron Source, High Flux Isotope Reactor User’s Group) executive committee. Served as Chair Fall 2003 thru 2004 http://www.sns.gov/shug/

Member (appointed), Oak Ridge National Lab’s Structural and Molecular Biology Task Force

Member, Biology subcommittee for American Conference of Neutron Scattering, June 6-9, 2004

Session Chair, Proteins and Membranes, American Conference of Neutron Scattering, June 6-9, 2004

Member, Organizing/Planning committee for Neutron Scattering for Chemistry and the Chemistry/Biology Interface (NSF CHEM/Bio) Workshop Sept. 2003

Discussion Leader, Breakout Session for Neutron Scattering for Chemistry and the Chemistry/Biology Interface (NSF CHEM/Bio) Workshop

Panelist, NSF Site Review

Reviewer, NSF Chemical Instrumentation Panel

Departmental Committees

Chair, Biochemistry Search Committee 2007-08

Nanoscale Science Ph. D. Planning Committee (2005 – present)

Physical Chemistry Search Committee (2004-05)

Space Committee (2002- present)

Graduate Committee (2000 – 2002)

Web Page Development Committee (2000 – present)

Honors Committee (1999 – present)

M.S. Thesis Committee (twenty students)

College/University Committees

Member (elected), Faculty Competitive Grants Committee (2007-09)

Panelist; UNC Charlotte CAREER Workshop lead by Lesley A. Brown, Director of Proposal Development April 5, 2005

Member, Comprehensive Review Committee for the Chair of the Department of Chemistry (2003)

Ph. D. in Biology Graduate Committee (2002- present)

Molecular Biology Search Committee (2001-02)

Ph. D. in Biotechnology Thesis Committee (Michael York, Ph. D. *07)

Ph. D. in Biotechnology Thesis Committee (Nathan Hedrick, Ph.D.*05)

Ph. D. in Biotechnology Thesis Committee (Rachael Huang, M.A. *06))

Ph. D. in Biotechnology Thesis Committee (Mingxuan Sun, M.S *07))

Ph. D. in Biotechnology Thesis Committee (Tiffany Epps)

Ph. D. in Biotechnology Thesis Committee (Monalisa Sunkara)

Ph. D. in Biotechnology Thesis Committee (Rana Zohby)

Seminar Program Host

Mar. 1, 2004 Dr. James Geiger, Michigan State University ““Mechanism and Allosteric Regulation of ADP-Glucose Pyrophosphorylase, the Rate-Limiting Step in Starch Biosynthesis”

Dec. 2, 2002 Dr. John Miller, Western Michigan University “Surface Photoreactions: Structure And Dynamics In Radical Abstractions And Electron Transfer Substitutions”

Mar. 26, 2001 Dr. Stephan Franzen, Chemistry Department NC State, "New Methods for Observation of DNA Hybridization on Surfaces"

Nov. 5, 2001 Bruce Hudson, Chemistry Department Syracuse University, “Vibrational Spectroscopy with Neutrons: testing ab Initio Methods”

Reviewing Activities

· Reviewer for FEBS letters

· Reviewer for Biochemistry

· Reviewer for Cell Biochemistry and Biophysics

· Reviewer for Biophysical Journal

· Reviewer for Journal of Applied Crystallography

· Reviewer for Journal of Biological Chemistry

· Referee for user proposals submitted to the National Institute for Standards and Technology Center for Neutron Research

· NSF Site Review Panelist, Chemical Instrument Grant Panelist, as well as numerous individual Proposal Reviews

· Research Corporation Proposal Review

· Department of Energy/Basic Energy Sciences Panel Review of LANCE, March 2006

· Department of Energy/Basic Energy Sciences Panel Review of HFIR, 2006

Outreach, Recruiting and Promotion Activities

· Contributed to Neutron Pulse Publications, SNS communications.

· Davidson IB Middle School Science Olympiad Coach for Awesome Aquifers Event 2005-06

· Chemistry Demonstrations for the Cherokee Girls visit to Math Camp at UNC Charlotte June 22, 2004

· Participated in the Charlotte Research Institute’s presentation to Duke Power on January 21, 2004

· Judge of oral presentations at the 2003 Junior Science & Humanities Symposium (JSHS) February 24, 2003.

· Advisor and Job Shadow Mentor for High School student’s project on biochemistry profession at Gaston Christian School (January 2003).

· Co-leader of Villa Heights Elementary School’s Science Club (2002-2005).

· Particpated in the Advisory Budget Commission’s visit to UNC Charlotte with Provost Denise Trauth, Chancellor James Woodward and Assistant to the Chancellor, James Dixon.

· External reviewer, by invitation, Center for Structural Molecular Biology Users' Facility Meeting, Oak Ridge National Laboratory, April 10 -11, 2000.

Bibliography

Ashish, Matthew S. Paine, Paul B. Perryman, Lin Yan, Helen L. Yin, and Joanna K. Krueger. (2007) “Global Structure Changes Assoicated with Ca2+ Activation of Full-length Human Plasma Gelsolin” J. Biol. Chem. 282(35): 25884-25892.

Fengwei Bai, Terrence Town, Deepti Pradhan, Jonathan Cox, Ashish, Michel Ledizet, John F. Anderson, Richard A. Flavell, Joanna K. Krueger, Raymond A. Koski, and Erol Fikrig. (2007) "Antiviral peptides targeting the West Nile Envelope Protein" J. Virology. 81(4)::2047-2055.

Garg, Renu; Juncadella, Ignacio J.; Ramamoorthi, Nandhini; Ashish; Ananthanarayanan, Shobana K.; Thomas, Venetta; Rincon, Mercedes; Krueger, Joanna K.; Fikrig, Erol; Yengo, Christopher M.; Anguita, Juan. (2006) “Cutting Edge: CD4 Is the Receptor for the Tick Saliva Immunosuppressor, Salp15”. J. Immunology 177(10): 6579-6583.

Gautam, J.K.; Ashish; Comeau, L.D., Krueger, J.K., and Smith, M.F. Jr. (2006) “Structural and Functional Evidence for the role of the TLR2 DD loop in TLR1/TLR2 Heterodimerization and Signaling”. J. Biol. Chem. 281(40):30132-42.

Ashish, Garg, R.; Anguita, J.; Krueger, J.K. (2006) “Binding of Full-length HIV-1 gp120 to CD4 Induces Structural Reorientation Around the gp120 Core”. Biophys. J. 91(6): L69-71.

Krueger, J. K. and Wignall, G. D. (2005) “Small Angle Neutron Scattering from Biological Molecules”. In Neutrons in Biology - Techniques and Applications ( J. Fitter, T. Gutberlet, J. Katsaras, Eds.) Springer Publications Biophysical Studies Series Chapter 8 pp. 127-160.

Heller, W. T., Krueger, J. K., and Trewhella, J. (2003) “Further Insights into Calmodulin-Myosin Light Chain Kinase Interaction from Solution Scattering and Shape Restoration” Biochemistry 42(36): 10579-588.

Krueger, J. K., Gallagher, S. C., Zhi, G., Geguchadze, R., Persechini, A., Stull, J. and Trewhella, J. (2001, accelerated publication) “Activation of Myosin Light Chain Kinase Requires Translocation of Bound Calmodulin” J. Biol. Chem. 276(7): 4535-4538.

Trewhella, J., and J. K. Krueger. (2001) “Small-angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium Binding Proteins and in Their Interactions with Regulatory Targets”. In Methods of Molecular Biology. (H.J. Vogel, Ed.) Vol 173, pp. 137 – 160. Humana Press.

Krueger, J. K., Gallagher, S. C., Wang, A., and Trewhella, J. (2000) “Calmodulin Remains Extended upon Binding to Smooth Muscle Caldesmon: A Combined Small-Angle Scattering and Fourier Transform Infrared Spectroscopy Study.” Biochemistry, 39(14): 3979-3987.

Krueger, J., McCrary, B., Wang, A. H.-J., Shriver, J. W., Trewhella, J., and Edmondson, S. P. (1999) “Solution Structure of Sac7d/DNA Complex Studied by Small-Angle X-ray Scattering”. Biochemistry 38(32): 10247-10255.

Krueger, J. K., Bishop, N. A., Blumenthal, D. K., Zhi, G., Beckingham, K., Stull, J. and Trewhella, J. (1998) “Calmodulin Binding to Myosin Light Chain Kinase Begins at Substoichiometric Ca²⁺ Concentrations: A Small-Angle Scattering Study of Binding and Conformational Transitions” Biochemistry 37(51): 17810-17817.

Stull, J. T., Lin, P. J., Krueger, J. K., Trewhella, J., and Zhi, G. (1998) “Myosin Light Chain Kinase: Functional Domains and Structural Motifs”. Acta Physiol. Scand. 164: 471-482.

Improta, S., Krueger, J. K., Gautel, M., Atkinson, R., Lefevre, J.-F., Moulton, S., Trewhella, J. and Pastore, A. (1998) “The Assembly of Immunoglobulin-like Modules in Titin: Implications For Muscle Elasticity”. J. Mol. Biol.